The mechanisms underlying the effects of AMH on Müllerian duct regression inmale mice

The Journal of Veterinary Medical Science

Takuya OMOTEHARA¹  Tetsushi HIRANO²  Anzu YAMAMOTO³  Tadashi TAKADA³  Yuuka MIURA³  Naoki YONEDA³  Nobuhiko HOSHI³  Toshifumi YOKOYAMA³  Youhei MANTANI⁴  Hiroshi KITAGAWA⁴ 

[1] Department of Anatomy, Tokyo Medical University, 6-1-1Shinjuku, Shinjuku, Tokyo 160-8402, Japan;Division of Drug and Structural Research, Life ScienceResearch Center, University of Toyama, 2630 Sugitani, Toyama 930-0194,Japan;Laboratory of Animal Molecular Morphology, Departmentof Animal Science, Graduate School of Agricultural Science, Kobe University, 1-1Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan;Laboratory of Histophysiology, Department of AnimalScience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada,Kobe, Hyogo 657-8501, Japan

关键词: AMH (anti-Müllerian hormone);    Müllerian duct regression;    secretion manner;    sexual differentiation;    true hermaphrodite;   

DOI  :  10.1292/jvms.18-0023

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Anti-Müllerian hormone (AMH) produced in the developing testis induces the regression ofthe Müllerian duct, which develops into the oviducts, uterus and upper vagina. In our truehermaphrodite mouse with an ovary on one side and a testis on the other (O/T), the oviductand uterus are present only on the ovary side, and nothing derived from the Müllerian ductis present on the testis side. Here, we investigate the mechanism underlying theunilateral Müllerian duct regression and the mode of AMH signaling, by performingimmunohistology, Western blotting, and organ culture analyses. The histological analysisrevealed that during the start of the Müllerian duct regression, the duct in the O/T micewas clearly regressed on the AMH-positive testis side compared to the AMH-negative ovaryside. The immunohistochemistry showed a diffuse immunoreaction of AMH in the interstitiumsurrounding the testis cord and boundary region between the testis and mesonephros,especially in the cranial portion. Western blotting revealed that the amount of AMH in thecranial half of the mesonephros was larger than that in the caudal half. AMH injected intothe gonads in organ culture induced the regression of the Müllerian duct via theinterstitium of the organ. These results suggest that AMH acts on the Müllerian duct inmale mice by exuding into the interstitium surrounding the testis cord and infiltratingthrough the cranial region from the testis to the mesonephros.

【 授权许可】

Unknown