Optical investigations reveal the effects of 2-aminoethyldiphenyl borate on STIM1 puncta formation

Journal of Innovative Optical Health Sciences

关键词: SOCE;    CRAC;    2-aminoethyldiphenyl borate;    STIM1;    Orai1;    confocal imaging;    FRET;   

DOI  :  10.1142/S1793545818500037

学科分类：医学（综合）

来源: World Scientific Publishing Co. Pte. Ltd.

【 摘 要 】

2-Aminoethyldiphenyl borate (2-APB) is the most commonly used pharmacological agent in the study of calcium release-activated channels (CRACs); however, its inhibitory mechanism to CRACs remains unclear. To address this issue, we systematically employed confocal imaging, dual-wavelength excitation photometry and FRET to examine the effects of 2-APB on the dynamic activities and function of STIM1 and Orai1, two key components of CRACs. Imaging results support that there are two signaling pathways (Orai1-independent and Orai1-dependent) for the formation of STIM1 puncta. 2-APB could dose dependently block Orai1-independent but not Orai1-dependent STIM1 puncta formation, despite its obvious inhibition effect on store-operated Ca2+ entry (SOCE). In addition, we found that although 2-APB could not visibly alter near plasma membrane CAD-eYFP localization, it could completely block CAD-YFP-induced constitutive Ca2+ entry and promote the interaction between Orai1 and CAD by FRET measurements. Therefore, we propos...

【 授权许可】

Unknown