Store‐operated Ca2+ signaling in dendritic cells occurs independently of STIM1

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: CRAC;    SOCE;    STIM2;    Orai2;   

DOI  :  10.1189/jlb.0610381

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

SOCEviaCRACchannelsisacriticalsignalingeventinimmunecells.Recentstudieshaveidentifiedkeyproteinsunderlyingthisprocess;STIMisanERCa2+sensorthatinteractswithOrai,anintrinsic,pore‐formingproteinoftheCRACchannel.Inheterologousexpressionsystems,STIM1regulatesSOCEbyinteractingwithOrai1,‐2,and‐3.Innativetissues,however,thepreciserolesofSTIMandOraiproteinsarenotwelldefined.Here,wehaveinvestigatedthemolecularcomponentsofSOCEsignalinginmouseDCs.WeshowthatDCspredominantlyexpressSTIM2andonlyverylowlevelsofSTIM1comparedwithTlymphocytes.UponstoredepletionwithTg,STIM2aggregatesandinteractsselectivelywithOrai2.Incontrast,TgfailstoaggregateSTIM1orenhanceSTIM1‐mediatedinteractionswithOraiproteins.Consistentwiththisbiochemicalcharacterization,stimulationofDCswiththeadhesionmoleculeICAM‐1selectivelyrecruitsSTIM2andOrai2totheIS.Together,thesedatademonstrateanovel,STIM2‐dependentSOCEsignalingpathwayinDCs...

【 授权许可】

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