Role of Ketotifen on metabolic profiles, inflammation and oxidative stress in diabetic rats

Endocrine journal

Zimiao Chen¹  Jian Wang²  Xingbo Cheng²  Qiong Chen³  Xuejiang Gu⁵  Liangshan Mu⁷  Hongwei Sun⁷  Shengjie Ge⁷  Liansong Ni⁷ 

[1] Department of Endocrinology, The First Affiliated Hospital of Soochow University, Suzhou 215000, People’Department of Endocrinology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’Department of Hand Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’Department of Neurosurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’s Republic of China

关键词: Ketotifen;    Diabetes mellitus;    Mast cell;    Inflammation;    Dyslipidemia;   

DOI  :  10.1507/endocrj.EJ16-0458

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

We aim to explore effects of Ketotifen on metabolic profiles, inflammation and oxidative stress.Sprague Dawley (SD) male rats were randomly divided into normal control group (NC) and experimental groups, and experimental group rats were fed with high-sugar and fat diet for 6 weeks.Then, experimental group rats were divided into diabetes group (DM) and ketotifen treatment group (KT).KT group was given ketotifen via Intragastric for 8 weeks with the dosage of 0.09 mg/kg/d.Fasting plasma glucose (FPG) was measured using glucose oxidase-phenol amino phenazone method.Fasting insulin (FINS), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were tested by enzyme-linked immunosorbent assay.Malondialdehyde (MDA) and superoxide dismutase (SOD) were quantified by spectrophotometer method.Before Ketotifen administration, compared with NC group, DM and KT groups showed significantly high levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α and MDA, and lower levels of HDL and SOD (All p <0.05).After 4 weeks of Ketotifen administration, levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α in KT group decreased significantly, and levels of HDL and SOD elevated significantly (All p <0.05).After 8 weeks of Ketotifen administration, levels of body weight, FPG, FINS, HOMA-IR, TC, TG, LDL, IL-6, TNF-α and MDA in KT group decreased more obviously, and levels of HDL and SOD elevated significantly further (All p <0.05).Ketotifen improved metabolic profiles, and ameliorated status of inflammation and oxidative stress.

【 授权许可】

Unknown