Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
NK1.1+ cells promote sustained tissue injury and inflammation after trauma with hemorrhagic shock | |
Loughran, Patricia1  Demetris, Anthony J.1  Scott, Melanie1  Billiar, Timothy R.5  Manson, Joanna5  Hoffman, Rosemary A.5  Ramadan, Mostafa5  | |
[1] and;..Clinical Translational Medical Center of Vascular Disease of the Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China;Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA;Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA | |
关键词: natural killer cell; T cell; leukocytes; cytokines; | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
Various cell populations expressing NK1.1 contribute to innate host defense and systemic inflammatory responses, but their role in hemorrhagic shock and trauma remains uncertain. NK1.1+ cells were depleted by i.p. administration of anti-NK1.1 (or isotype control) on two consecutive days, followed by hemorrhagic shock with resuscitation and peripheral tissue trauma (HS/T). The plasma levels of IL-6, MCP-1, alanine transaminase (ALT), and aspartate aminotransferase (AST) were measured at 6 and 24 h. Histology in liver and gut were examined at 6 and 24 h. The number of NK cells, NKT cells, neutrophils, and macrophages in liver, as well as intracellular staining for TNF-α, IFN-γ, and MCP-1 in liver cell populations were determined by flow cytometry. Control mice subjected to HS/T exhibited end organ damage manifested by marked increases in circulating ALT, AST, and MCP-1 levels, as well as histologic evidence of hepatic necrosis and gut injury. Although NK1.1+ cell–depleted mice exhibited a similar degree of organ damage as nondepleted animals at 6 h, NK1.1+ cell depletion resulted in marked suppression of both liver and gut injury by 24 h after HS/T. These findings indicate that NK1.1+ cells contribute to the persistence of inflammation leading to end organ damage in the liver and gut.
【 授权许可】
CC BY
【 预 览 】
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RO201902186870127ZK.pdf | 80KB | download |