Simultaneous determination of thimerosal and aluminum in vaccines and pharmaceuticals with the use of HPLC method

Acta Chromatographicae

R. Rawski¹  P.T. Sanecki¹  M. Zaręba² 

[1] Department of General Chemistry and Electrochemistry, Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, Poland;Department of General Chemistry and Electrochemistry, Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzeszow, Poland E-mail: [e-mail]

关键词: thimerosal;    aluminum;    HPLC;    dithizone;    vaccines;   

DOI  :  10.1556/1326.2016.28.3.2

学科分类：化学（综合）

来源: Akademiai Kiado Rt.

【 摘 要 】

A simple and convenient chromatographic method of simultaneous separation, identification, and quantitative determination of thimerosal (TM) (preservative) and aluminum (adjuvant) in vaccines and pharmaceuticals by reversed phase highperformance liquid chromatography (RP-HPLC) with visible (VIS) detection was developed and validated. Due to postcolumn derivatization with dithizone, any interference from matrix was excluded. Similarly, a possibility of on-column decomposition of dithizonates was eliminated. Evaluated detection limits were 0.3 μg TM and 3.0 μg Al, which correspond to the smallest, but possible to recognize, visible peak.

【 授权许可】

Unknown