Evaluation of biofilm-forming ability of bacterial strains isolated from the roof of an old house

The Journal of General and Applied Microbiology

Rahma Laadhar¹  Sami Aifa¹  Faten Abdelli¹  Sami Mnif¹  Marwa Jardak²  Raphael Lami³  Didier Stien³ 

[1] Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax;Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax . Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique;Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique

关键词: biofilm;    crystal violet;    resazurin;    Staphylococcus epidermidis;   

DOI  :  10.2323/jgam.2016.10.005

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The bacterial diversity associated with biofilm-forming ability was studied. Eighteen bacterial strains were isolated from a microbial film collected from the roof of an old house located in Sfax, Tunisia. The purity of these microorganisms was confirmed by microscopic observation after repeated streaking on a Tryptic Soy agar medium. Biofilm formation was estimated using preliminary tests including a motility test, microbial adhesion to solvents (MATS), and the Congo Red Agar method (CRA). Since these tests showed no significant result, microplate tests, such as crystal violet and resazurin assays, were used. The results obtained showed that strain S61 was able to form a biofilm within 24 h (OD570 = 4.87). The viability of the S61 biofilm with resazurin assessed with fluorescence measurement was about 1.5 × 103. The S61 strain was identified as Staphylococcus epidermidis. In the biofilm studied here, it was the most biofilm-forming bacterium and will be used as a bacterial model for studying anti-biofilm activity.

【 授权许可】

Unknown