Shiga Toxin Subtypes and Virulence Genes in Escherichia coli Isolated from Cattle

Japanese journal of infectious diseases

Hiroshi Sakae¹  Masaharu Fukunaga¹  Hidetaka Tsuji¹  Yumi Akiyama²  Etsuko Saito²  Masatsugu Chikahira²  Kenichi Ogita²  Hiroko Futai²  Atsushi Iguchi² 

[1] Hyogo Prefectural Meat Inspection Center;Public Health Science Research Center, Hyogo Prefectural Institute of Public Health and Consumer Sciences

关键词: Shiga toxin;    subtype;    Escherichia coli;    virulence gene;    cattle;   

DOI  :  10.7883/yoken.JJID.2016.100

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Subtypes of stx1 and stx2 in 45 Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle were investigated by PCR. Only subtype stx1a was detected among all the stx1-positive strains. The major stx2 subtype was stx2a followed by stx2d, stx2c, stx2b, and stx2g in decreasing order of frequency. stx2c was found in strains of serotypes O157 and O174. stx2d was found in 11 strains. These strains were confirmed by DNA sequencing to carry both the activatable tail and the END motif; all were eae-negative, and 3 contained stx2d as the only stx. stx2g was found in 2 strains in association with stx2a, estA1, and astA. In addition, 7 hybrid strains of shigatoxigenic and enterotoxigenic E. coli (STEC/ETEC) were found to harbor one or both of stx1a and stx2a (stx1a/stx2a) and estA1. Among 27 serotypes of STEC strains isolated from cattle, O157:H7 and O109:H– strains were eae-positive. Other putative adhesin genes, such as saa, iha, espP, and lpfAO113 were detected in more than 12 serotypes.

【 授权许可】

Unknown