Callus induction and plant regeneration of Michelia champaca (Magnoliaceae): A multipurpose tree

Dynamic Chiropractic

A. H. A. Abdelmageed¹  Faridah Q. Z.²  Nor Shuhada K.³ 

[1] Biodiversity Unit, Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.;Department of Biology, Faculty of Science, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.;Department of Horticulture, University of Khartoum, Shambat 13314, Khartoum North, Sudan.

关键词: Michelia champaca;    Tissue culture;    Callus;    Axillary bud explant;    Medicinal plant;    Plant growth regulators.;   

DOI  :  10.5897/JMPR12.196

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Michelia champacacallus was induced from axillary bud explants cultured onto Murashige and Skoog (MS) basal medium supplemented with different concentrations of auxins (IAA and 2,4-D) and cytokinin (BAP) as plant growth regulators. It was observed that callus ofM. champacacould be induced on MS media supplemented with 0.0 - 2.0 mg/l IAA, 0.0 - 2.0 mg/l 2,4-D, and 0.0 - 2.0 mg/l BAP, respectively. All growth regulators that were used in this study are capable of inducing callus from explants. 0.10 mg/l IAA and 0.10 mg/l 2,4-D were found to be the suitable concentrations for the induction of callus. The survival rate of explants in the media supplemented with IAA was found to be 81%. MS Media supplemented with different concentrations of BAP combined with IAA and 2,4-D were tested for callus proliferation. The callus growth showed good response to all growth regulators combinations tested in this study, except the combination BAP/IAA with 0.2/0.10 mg/I which exhibited low response with dark brown callus. The combination 2.00 mg/l BAP with 0.50 mg/l IAA and all the combinations of BAP with 2,4-D showed the highest rate of callus proliferation with cream colour. This study suggests that auxins along with cytokinins have a critical role on the callus and shoot proliferation of this plant.

【 授权许可】

Unknown