Identification of Group G Streptococcal Isolates from Companion Animals in Japan and Their Antimicrobial Resistance Patterns

Japanese journal of infectious diseases

Goro Kurita¹  Yoshiteru Murata²  Takashi Takahashi³  Yuzo Tsuyuki³  Mieko Goto⁴ 

[1] Division of Clinical Laboratory, Sanritsu Zelkova Veterinary Laboratory;Kurita Animal Hospital;Laboratory of Infectious Diseases, Kitasato Institute for Life Sciences, Kitasato University;Murata Animal Hospital

关键词: identification;    antimicrobial resistance;    group G streptococci;    companion animals;    Japan;   

DOI  :  10.7883/yoken.JJID.2016.375

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

In this study, we conducted a species-level identification of group G streptococcal (GGS) isolates from companion animals in Japan and analyzed antimicrobial resistance (AMR) patterns. Strains were isolated from sterile and non-sterile specimens collected from 72 animals with clinical signs or symptoms in April-May, 2015. We identified the strain by 16S rRNA sequencing, mass spectrometry (MS), and an automated method based on their biochemical properties. Antimicrobial susceptibility was determined using the broth microdilution method and E-test. AMR determinants (erm(A), erm(B), mef(A), tet(M), tet(O), tet(K), tet(L), and tet(S)) in corresponding resistant isolates were amplified by PCR. The 16S rRNA sequencing identified the GGS species as Streptococcus canis (n = 68), Streptococcus dysgalactiae subsp. equisimilis (n = 3), and S. dysgalactiae subsp. dysgalactiae (n = 1). However, there were discrepancies between the sequencing data and both the MS and automated identification data. MS and the automated biochemical technique identified 18 and 37 of the 68 sequencing-identified S. canis strains, respectively. The AMR rates were 20.8% for tetracycline and 5.6% for clarithromycin, with minimum inhibitory concentrations (MIC)50 -MIC90 of 2-64 and ≤ 0.12-0.25μg/mL, respectively. AMR genotyping showed single or combined genotypes: erm(B) or tet(M)-tet(O)-tet(S). Our findings show the unique characteristics of GGS isolates from companion animals in Japan in terms of species-level identification and AMR patterns.

【 授权许可】

Unknown