Evaluation of comparative antioxidant potential of aqueous and organic fractions of Ipomoea carnea

Dynamic Chiropractic

关键词: Ipomoea carneaJacq.;    DPPH assay;    total antioxidant activity;    FRAP value;    total phenolics;    Inhibition of lipid peroxidation (%).;   

DOI  :  10.5897/JMPR10.287

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The methanolic extract ofIpomoea carneaJacq. was dissolved in distilled water and partitioned withn-hexane, chloroform, ethyl acetate andn-butanol successively. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: DPPH free radical scavenging activity, total antioxidant activity, FRAP assay and ferric thiocyanate assay and total phonolics were also determined. All the fractions showed significant antioxidant potential. The results revealed thatn-butanol fraction showed highest value of % inhibition of DPPH radical (91.11% ± 0.68),IC50ofn-butanol fraction was 74.65 ± 1.4 µg/ml, relative to butylated hydroxytoluene (BHT), havingIC50of 12.1 ± 0.92mg/mL. The chloroform soluble fraction showed highest total antioxidant activity (0.9096 ± 0.1) and highest total phenolic contents (113.05 ± 1.2 mg of gallic acid equivalents) as compared to other fractions. The ethyl acetate soluble fraction exhibited highest FRAP value (511.99 ± 1.8 µg of trolox equivalents) as well as highest value of inhibition of lipid peroxidation (61.87% ± 1.2) as compared to other fractions.

【 授权许可】

Unknown