Colebrookia oppositifolia: A valuable source for natural antioxidants

Dynamic Chiropractic

Muhammad Athar Abbasi¹  Tauheeda Riaz² 

[1] Department of Botany, Government College University, Lahore-54000, Pakistan.;Department of Chemistry, Government College University, Lahore-54000, Pakistan.

关键词: Colebrookia oppositifoliaSmith;    DPPH assay;    total antioxidant activity;    FRAP value;    total phenolics;    Inhibition of lipid peroxidation (%).;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Methanolic extract ofColebrookia oppositifoliaSmith. was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sequentially. Phytochemical screening showed the presence of phenolics, flavonoides and cardiac glycosides in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The polar fractions showed noteworthy antioxidant potential. The results revealed that ethyl acetate soluble fraction showed the highest value of percentage inhibition of DPPH (83.43 ± 1.27) at concentration of 60 µg/ml. The IC50of this fraction was 27.2 ± 0.23 µg/ml, relative to butylated hydroxytoluene (BHT), having IC50of 12.1 ± 0.92 µg/mL. It also showed highest FRAP value (172.23 ± 1.66 µg of trolox equivalents), as well as highest value of inhibition of lipid peroxidation (57.61 ± 1.1%) as compared to the studied fractions. The chloroform fraction showed highest total antioxidant activity (0.711 ± 0.031), as well as highest total phenolic contents (72.5 ± 0.34).

【 授权许可】

Unknown