In vitro reversal of deformity and inhibition of aggregation of sickle red blood cells by two Congolese herbal medicines

Dynamic Chiropractic

Chika Yamamoto¹  Jose Nzunzu Lami²  Marie Miezi Nsimba³  Toshiyuki Kaji⁴ 

[1]Centre de Phytothé

[2]Laboratory of Bio-Organic Research, Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Kinshasa University, P. O. Box 212 Kinshasa XI, D. R. Congo.

[3]Organization for Frontier Research and Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa 920-1181, Japan.

[4]rapie Moderne Nieca, Kinshasa, D. R. Congo.

关键词: Sickle cell anemia (SCA);    red blood cell (RBC);    deformity;    hypoxia;    aggregation;    hemoglobin S;    hemoglobin A;    blood viscosity;    hemolysis;    Ceiba pentandra;    Quassia africanaBaill.;   

DOI  :  10.5897/JMPR11.376

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The effects of aqueous extracts from the trunk bark and branches ofCeiba pentandraand from the root bark ofQuassia africanaBaill., which are claimed to overcome the clinical events of the sickle cell anemia (SCA) in Democratic Republic of Congo (DRC), were investigatedin vitroon the red blood cells (RBC) deformity and aggregation. Blood samples from SCA patients and from healthy persons were treated with 2% sodium metabisulfite to induce hypoxia and sickling of erythrocytes, and then, were incubated with the drug extracts. It was found that extracts, used separately or together, reversed the induced deformity of RBC. On the other hand, aggregates of RBC were incubated with the plant extracts and the action was evaluated by microscope examination, which showed that cells became dispersed and isolated, while they remained stacked in the samples not treated. Sickling of RBC is a major factor among others, which are implicated for initiating the events of sickle cell crises as well as the increasing red blood cells adhesiveness observed in increased blood viscosity. These observations could support the use of the two medicinal drugs to deal with the clinical events of SCA.

【 授权许可】

Unknown