Separation and purification of chlorogenic acid from Lonicera japonica Thunb. leaves exact with macroporous resins

Dynamic Chiropractic

Jun Zhang¹  Shujun Zhang² 

[1] College of Life Sciences, Northwest A and F University, Yangling, Shaanxi 712100, China.;College of Science, Northwest A and F University, Yangling, Shaanxi 712100, China.

关键词: Macroporous resins;    Lonicera japonicaThunb. Leaves;    chlorogenic acid;    adsorption;    desorption ratios.;   

DOI  :  10.5897/JMPR11.630

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Chlorogenic aicd is a main bioactive constituent inLonicera japonicaThunb. leaves. In this study, a simple method for separation and purification of chlorogenic acid fromL. japonicaleaves was established with macroporous resins. The performance and adsorption characteristics of four macroporous resins had been evaluated and D-101 was affirmed to be the suitable resin for the separation of chlorogenic acid. Dynamic adsorption and desorption tests performed in a column packed with D-101 resin were followed to optimize the process parameters. The final results showed that the highest purity of the chlorogenic acid product was 50.72% with a recovery yield of 54.44% when the parameters were as follows: a sample concentration in solution of 1.01 mg/mL chlorogenic acid, a pH value of 4, a flow rate of 2 bed volume (BV)/h, a sample volume of 5 BV and an elution solvent of 20% (v/v) ethanol.

【 授权许可】

Unknown