Neisseria gonorrhoeae Challenge Increases Matrix Metalloproteinase-8 Expression in Fallopian Tube Explants

Frontiers in Cellular and Infection Microbiology

Muñ¹  Christodoulides, Myron¹  Solar, Paula³  Rodas, Paula I.³  Juica, Natalia E.³  Velasquez, Luis A.⁵  Borda, Paula⁵  oz, Cristobal⁵  Vargas, Renato⁷  Paredes, Rodolfo⁷ 

[1] , Santiago, Chile;Facultad de EcologíFacultad de Medicina, Center for Integrative Medicine and Innovative Science, Universidad Andres Bello, Santiago, Chile;Neisseria Research Group, Sir Henry Wellcome Laboratories, Division of Infection, Inflammation and Immunity, University of Southampton Medical School, Southampton, United Kingdom;Servicio de Ginecología y Obstetricia, Clía y Obstetricia, Hospital San Joséa y Recursos Naturales, Escuela de Medicina Veterinaria, Universidad Andres Bello, Santiago, Chile;nica Indisa, Santiago, Chile

关键词: Fallopian Tubes;    Neisseria gonorrhoeae;    Extracellular Matrix;    Metalloproteinases;    Mucosal damage;   

DOI  :  10.3389/fcimb.2017.00399

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Abstract Background Neisseria gonorrhoeae (Ngo) is the etiological agent of gonorrhea, a sexually transmitted infection that initially infects the female lower genital tract. In untreated women, the bacteria can ascend to the upper genital reproductive tract and infect the fallopian tube (FTs), which is associated with salpingitis and can lead to functional impairment of the FTs and infertility. The extracellular matrix (ECM) plays an important role in cell migration and differentiation in the female genital tract, and some pathogens have developed mechanisms to modify the ECM to establish successful infection. The ECM is regulated by matrix metalloproteinases (MMPs) and their endogenous inhibitors TIMPs, whereas MMPs deregulation can lead to pathological conditions in a variety of tissues. Results The aim of this work was to analyze the expression and localization of MMP-3, MMP-8, MMP-9 and TIMP-1 in FTs explants during Ngo infection using real-time PCR, immunohistochemistry, zymography and ELISA. No significant variation in the transcription level of MMP-3, MMP-9 and TIMP-1 was observed. In contrast, a significant transcript increase (p<0.05) was observed for MMP-8 that was accompanied by stromal immunoreactivity in infected explants. ELISA results supported these results where the release of MMP-8 was increased upon gonococcal infection. Conclusions Our results indicate that gonococcal infection induces an increase in MMP-8 expression, which may contribute to FTs damage during infection.

【 授权许可】

CC BY