Hysteroscopy and the butyl-cyanoacrylate on experimental sterilization of rabbit uterine tubes

Acta Cirurgica Brasileira

Henri Chaplin Rivoire²  Djalma José Fagundes¹  Sergio Bigolin¹  Anna Tereza Negrini Fagundes¹ 

[1] ,Head Rio Grande Federal Foundation University Department of Surgery Rio Grande RS ,Brazil

关键词: Sterilization;    Reproductive;    Tissue Adhesives;    Fallopian Tubes;    Rabbits;    Esterilização Reprodutiva;    Adesivos Teciduais;    Tubas Uterinas;    Coelhos;   

DOI  :  10.1590/S0102-86502007000500012

来源: SciELO

【 摘 要 】

PURPOSE: To assess the sterilization effectiveness on uterine tube of rabbit by the cyanoacrylate adhesive. METHODS: Hysteroscopy tubal catheterization was performed randomly in 12 animals (24 uterine tubes) assigned to the sham group (GS) and 15 animals (30 uterine tubes) to the n-butyl-cyanoacrylate (GB). The female rabbits were observed during 30, 90 and 180 days and mated to fertile males. The no pregnant rabbits were submitted to in vitro burst pressure test for patency by air insufflation (40 mmHg). The microscopic assessment was performed to parameters of damages in epithelium caused by the adhesive, the degree of inflammatory process, morphometry data values of tube diameter (UT) (cm), mucosa thickness (MT) and the myosalpinx thickness (MyT) (mm). The mucosa cells densitometry (total optical density) was expressed by the amount of DNA. The significance of the differences in histological scores and in thickness measurements were made by ANOVA test (P value < 0.05). RESULTS: In all animals of GB: the adhesive was attached to the mucosa; there was no pregnancy; no records of significant degree on inflammatory process; the patency test was negative and densitometry of DNA showed similar values to the both groups independently of observation periods. The layers thickness of GB-UT(1.118±0.117), GB-MT(0.447±0.247) and GB-MyT(0.853±0.097) were larger than the GS-UT(0.666±0.409), GS-MT(0.211±0.070) and GS-MyT(0.442±0.143). CONCLUSION: This approach offers a safe and feasible method of uterine tube obstruction.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License