Standardization of protocol for in vitro propagation of an endangered medicinal plant Rauwolfia serpentina Benth.

Dynamic Chiropractic

关键词: Rauwolfia serpentina;    in vitroculture;    naphthalene acetic acid;    benzyl adenine;    indole buteric acid;    MS medium.;   

DOI  :  10.5897/JMPR11.066

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The present investigation was carried out with a view to standardize anin vitroculture technique for mass propagation of an endangered medicinal plantRauwolfia serpentinaBenth. Shoot tip was used as explant for initial culture. The explants were cultured on MS (Murashige and Skoog, 1962) medium supplemented with different concentration and combination of NAA and BA for primary shoot proliferation. The best shoot proliferation was observed in MS medium containing 0.1 mg L-1NAA and 2.5 mgL-1BA, where 92% of plants showed proliferation. For rooting, half strength MS medium supplemented with 0.4 mgL-1NAA and 0.1 mgL-1IBA showed maximum root formation (91%). After acclimatization and transplantation, 90% of thein vitroderived plants survived inex vivocondition.

【 授权许可】

Unknown