Molecular Characterization of the Human Stomach Microbiota in Gastric Cancer Patients

Frontiers in Cellular and Infection Microbiology

Goldstein, Alisa M.²  Ding, Ti²  Humphrys, Michael S.³  Yu, Guoqin⁴  Taylor, Philip R.⁵  Ravel, Jacques⁵  Hu, Nan⁵  Abnet, Christian C.⁶  Medrano-Guzman, Rafael⁷  Torres, Javier⁸  Herrera-Goepfert, Roberto⁹  Wang, Lemin^1,10  Wang, Chaoyu^1,11 

[1] Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States;Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, United States;Instituto Nacional de CancerologíIntegrative Tumor Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States;Metabolic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States;Shanxi Cancer Hospital, Taiyuan, China;Unidad Medica de Alta Especialidad OncologíUnidad de Investigacion en Enfermedades Infecciosas, Unidad Medica de Alta Especialidad Pediatria, Centro Medico Nacional SXXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico;a, Centro Medico Nacional SXXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico;a, Secretaria de Salúd, Mexico City, Mexico

关键词: Helicobacter pylori;    16S rRNA;    KEGG modules;    microbiome;    gastric cancer;   

DOI  :  10.3389/fcimb.2017.00302

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Helicobacter pylori (Hp) is the primary cause of gastric cancer but we know little of its relative abundance and other microbes in the stomach, especially at the time of gastric cancer diagnosis. Here we characterized the taxonomic and derived functional profiles of gastric microbiota in two different sets of gastric cancer patients, and compared them with microbial profiles in other body sites. Paired nonmalignant and tumor tissues were sampled from 160 gastric cancer patients with 80 from China and 80 from Mexico. The 16S rRNA gene V3-V4 region was sequenced using MiSeq platform for taxonomic profiles. PICRUSt was used to predict functional profiles. Human Microbiome Project was used for comparison. We showed that Hp is the most abundant member of gastric microbiota in both Chinese and Mexican samples (51% and 24% respectively), followed by oral-associated bacteria. Taxonomic (phylum-level) profiles of stomach microbiota resembled oral microbiota, especially when the Helicobacter reads were removed. The functional profiles of stomach microbiota, however, were distinct from those found in other body sites and had higher inter-subject dissimilarity. Gastric microbiota composition did not differ by Hp colonization status or stomach anatomic sites, but did differ between paired non-malignant and tumor tissues in either Chinese or Mexican samples. Our study showed that Hp is the dominant member of the non-malignant gastric tissue microbiota in many gastric cancer patients. Our results provide insights on the gastric microbiota composition and function in gastric cancer patients, which may have important clinical implications.

【 授权许可】

CC BY