In vitro and in vivo efficacy of non-psychoactive cannabidiol in neuroblastoma

Current oncology

R. Gallily²  R. Smoum³  A. Toren³  R. Mechoulam³  I. Moshe⁴  T. Fisher⁴  D. Waldman⁴  H. Golan⁴  S. PriChen⁵  N. Peshes-Yaloz⁵  A. Castiel⁵  G. Schiby⁵ 

[1] Tel-Aviv University;The Edmond and Lily Safra Children’s Hospital;Hebrew University of Jerusalem;Sheba Cancer Research Center;The Chaim Sheba Medical Center

关键词: Neuroblastoma;    cannabidiol;    Δ9-tetrahydrocannabinol;    apoptosis;    tumour xenograft models;    nonpsychoactive cannabinoids;   

DOI  :  10.3747/co.23.2893

学科分类：肿瘤学

来源: Multimed, Inc.

【 摘 要 】

BackgroundNeuroblastoma (nbl) is one of the most common solid cancers in children. Prognosis in advanced nbl is still poor despite aggressive multimodality therapy. Furthermore, survivors experience severe long-term multi-organ sequelae. Hence, the identification of new therapeutic strategies is of utmost importance. Cannabinoids and their derivatives have been used for years in folk medicine and later in the field of palliative care. Recently, they were found to show pharmacologic activity in cancer, including cytostatic, apoptotic, and antiangiogenic effects. MethodsWe investigated,in vitroandin vivo,the anti-nbl effect of the most active compounds inCannabis , Δ9-tetrahydrocannabinol (thc) and cannabidiol (cbd). We set out to experimentally determine the effects of those compounds on viability, invasiveness, cell cycle distribution, and programmed cell death in human nbl SK-N-SH cells. ResultsBoth compounds have antitumourigenic activityin vitroand impeded the growth of tumour xenograftsin vivo.Of the two cannabinoids tested, cbd was the more active. Treatment with cbd reduced the viability and invasiveness of treated tumour cellsin vitroand induced apoptosis (as demonstrated by morphology changes, sub-G1 cell accumulation, and annexin V assay). Moreover, cbd elicited an increase in activated caspase 3 in treated cells and tumour xenografts. ConclusionsOur results demonstrate the antitumourigenic action of cbd on nbl cells. Because cbd is a non psychoactive cannabinoid that appears to be devoid of side effects, our results support its exploitation as an effective anticancer drug in the management of nbl.

【 授权许可】

Unknown