In vitro antibacterial activities of crude extracts of Garcinia kola seeds against wound sepsis associated Staphylococcus strains

Dynamic Chiropractic

T. Sib¹  a² 

[1] Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa.;Discipline of Microbiology, School of Biochemistry, Genetics and Microbiology, University of KwaZulu-Natal (Westville campus), Durban, South Africa.

关键词: Staphylococcus;    wound sepsis;    Garcinia kola;    antistaphylococcal activity;    antibiotic potentiation.;   

DOI  :  10.5897/JMPR09.194

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Extracts ofGarcinia kolaseeds were evaluated for their activity against fourStaphylococcusstrains isolated from wound sepsis specimens. Three of the isolates were identified by 16S rDNA sequencing asStaphylococcusaureuswith the GenBank Accession numbers, EU244633, EU244634, EU244636, and another was identified asStaphylococcussciuri(Accession number EU244635). The aqueous methanol and acetone extracts ofGarcinia kolaseeds showed activity against all four isolates at 30 mgml-1(aqueous extract) and 10 mgml-1(acetone and methanol extracts). The MIC values for the aqueous extract were the same (10 mgml-1) for all the isolates. The acetone and methanol extracts had lower MIC values in the ranges of 0.3125 - 0.625 mgml-1. The acetone extract showed strong bactericidal activity againstS.aureusstrain OKOH3 resulting in a 2.70 Log10reduction in counts at 1.25 mgml-1(2 × MIC) within 4 h of exposure and a complete elimination of the organism after 8 h. The same extract was weakly bactericidal againstS.aureusstrain OKOH1, achieving only a 2.92 Log10reduction in counts at 1.25 mgml-1(4 × MIC) in 24 h. The interactions between the acetone extract and antibiotics were largely additive and indifferent with no combinations showing classical synergistic interactions. We conclude that extracts ofGarcinia kolaseeds can potentially be useful in the treatment of staphylococcal wound infections.

【 授权许可】

Unknown