Preparative purification of coniferyl ferulate from Angelica sinensis oil by high performance centrifugal partition chromatography

Dynamic Chiropractic

关键词: Angelica sinensis;    coniferyl ferulate;    high performance centrifugal partition chromatography;    solvent extraction.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

A high performance centrifugal partition chromatography (HPCPC) method was successfully developed to purify coniferyl ferulate (CF) from the extracts of commercialAngelica sinensisoil by usingn-hexane-ethyl acetate-ethanol-water (5:5:5:5, v/v) as the two-phase solvent system. The upper and lower phases of this system were used as stationary and mobile phases, respectively. The purity of coniferyl ferulate was over 98% as determined by high-performance liquid chromatography (HPLC). The structure of coniferyl ferulate was identified by MS,1H NMR and13C NMR.

【 授权许可】

Unknown