Real-Time Monitoring of Stem Cells by Diamond-Based Impedance Sensors

Proceedings

Procházka, Václav¹ 

关键词: diamond film;    biosensor;    stem cells;    impedance spectroscopy;   

DOI  :  10.3390/proceedings1040515

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with built-in gold interdigitated electrodes (IDEs) as a promising platform for simultaneous electrical and optical monitoring of adipose-derived stem cells (ASC). The impedance spectra were collected in a wide frequency range (from 100 Hz to 50 kHz) for 27 h of cultivation in chambers designed for static cultivation. Absolute impedance spectra were analyzed in terms of measurement frequencies and cell properties monitored by high-resolution digital camera.

【 授权许可】

CC BY