Antibody Modified Gold Electrode as an Impedimetric Biosensor for the Detection of Streptococcus pyogenes

Sensors

卷:20

Sabina Żołędowska¹  Elżbieta Czaczyk²  Natalia Malinowska²  Wioleta Białobrzeska²  Katarzyna Pałka²  Katarzyna Pala²  Tomasz Łęga²  Dawid Nidzworski²  Karolina Dziąbowska² 

[1] Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland;

[2] SensDx, 14b Postępu St., 02-676 Warszawa, Poland;

关键词: biosensor;    4-aminothiophenol;    gold electrode;    impedance spectroscopy;    antibodies;    Streptococcus pyogenes;   

DOI  :  10.3390/s20185324

来源: DOAJ

【 摘 要 】

Streptococcus pyogenes is a known cause of a wide spectrum of diseases, from mild and acute to severe invasive infections. This paper concerns the development of a novel impedimetric biosensor for the detection of the mentioned human pathogen. The proposed biosensor is a gold disk electrode modified with commercially available antibodies attached to the surface of the electrode by carbodiimide chemistry. The conducted tests confirmed the specificity of the antibodies used, which was also demonstrated by the results obtained during the detection of S. pyogenes using electrochemical impedance spectroscopy. The developed sensor successfully detected the presence of S. pyogenes in the sample and the detection limit was calculated as 9.3 cfu/mL. The results obtained show a wide linear range for verified concentrations of this pathogen in a sample from 4.2 × 10² to 4.2 × 10⁶ cfu/mL. Furthermore, the optimal experimentally determined time required to perform pathogen detection in the sample was estimated as 3 min, and the test did not lead to the degradation of the sample.

【 授权许可】

Unknown