Antifungal activity of stigmasterol, sitosterol and ergosterol from Bulbine natalensis Baker (Asphodelaceae)

Dynamic Chiropractic

关键词: Bulbine natalensis;    phytosterols;    secondary metabolites;    antifungal activity;    mycotoxins.;   

DOI  :  10.5897/JMPR12.151

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Bulbine natalensisBaker.(Asphodelaceae) is indigenous to only southern Africa and is widely used as a skin remedy.B. natalensiscontain secondary metabolites that have antibacterial properties. Phytosterols are a group of steroid alcohols and phytochemicals that occur naturally in plants.Aspergillus,PenicilliumandFusariumspecies are considered to be the most toxigenic fungi. They produce a large consortium of mycotoxins that include aflatoxins B1, B2, G1, G2,fumonisin B1and ochratoxins. They are found in foodstuffs and are not destroyed by normal industrial processing or cooking since they are heat-stable. The purpose of the project was to extract and quantify phytosterols, such as ergosterol, stigmasterol and sitosterol fromB. natalensisusing phytochemical methods such as thin layer chromatography and high performance liquid chromatography. The extract was tested againstAspergillus flavus, Penicillium digitatumandFusarium verticilloidesfor antifungal activities. 1 ml of saponified extract contained 0.07 µg of sitosterol, 0.16 µg of stigmasterol and 0.37 µg of ergosterol. Saponifiable, unsaponifiable and crude extracts showed minimal activity againstF. verticilloides, whereas potent activity was exhibited againstA. flavus.

【 授权许可】

Unknown