Termoestabilidad del ADN deshidratado de Mycoplasma gallisepticum

Revista de salud animal

Perez-Castillo, Anisleidys¹  Burger-Pulgarón, Yaima¹  Relova-Vento, Damarys¹  Lobo-Rivero, Evelyn¹  Duque-Ortiz, Arianna¹ 

[1] Centro Nacional de Sanidad Agropecuaria, San José de las Lajas, Cuba

关键词: DNA;    diagnostics;    PCR;    thermal stability.;   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

The preservation and storage of genetic material are of vital interest especially for mycoplasmas, since it is known that the traditional conservation methods reported for these species are not completely effective. The diagnosis of these microorganisms is carried out by standard and molecular methods; among the latter is the Polymerase Chain Reaction test (PCR). One of the critical points of this test is the stability of the deoxyribonucleic acid (DNA). The aim of this work was to evaluate the thermal stability of fragments amplified by PCR, from dehydrated DNA, from the 16S rRNA gene for the detection of Mollicutes and from the 16S rRNA and mgc2 genes for the diagnosis of M. gallisepticum, targets commonly used in diagnosis. The product of each extraction was dried and stored at 4, 20 and 37 ºC for a period of 90 days. The stability over time of the different regions of the diagnosis was assessed by amplification using PCR. The results showed that M. gallisepticum DNA was heat stable at room temperature for a period of 90 days. Furthermore, it was found that the purity of the extracted DNA did not affect its stability when kept dehydrated. The results showed that preserving the DNA of M. gallisepticum dehydrated allows storage and transfer without high costs and without affecting the quality of the samples for diagnosis

【 授权许可】

CC BY-NC