Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP)

FASEB BioAdvances

Jayme N. Poppin¹  Brandon M. Wagner¹  David Scott Copeland¹  Kevin D. Foley¹  Michelle M. Gray¹  Jordan T. Aken¹  John A. Pezza²  Nathan A. Tanner²  Nicole M. Nichols²  Kutay D. Atabay³  Julian Rubinfien⁴  Ezequiel Alvarez Saavedra⁵  Sebastian Kraves⁵  Emily J. Gleason⁵ 

[1] Boeing Defense Berkeley MI USA;New England Biolabs, Inc. Ipswich MA USA;Whitehead Institute for Biomedical ResearchMassachusetts Institute of Technology Cambridge MA USA;Yale University New Haven CT USA;miniPCR bio Cambridge MA USA;

关键词: diagnostics;    DNA amplification;    PCR;    space exploration;   

DOI  :  10.1096/fba.2019-00088

来源: DOAJ

【 摘 要 】

Abstract Human spaceflight endeavors present an opportunity to expand our presence beyond Earth. To this end, it is crucial to understand and diagnose effects of long‐term space travel on the human body. Developing tools for targeted, on‐site detection of specific DNA sequences will allow us to establish research and diagnostics platforms that will benefit space programs. We describe a simple DNA diagnostic method that utilizes colorimetric loop‐mediated isothermal amplification (LAMP) to enable detection of a repetitive telomeric DNA sequence in as little as 30 minutes. A proof of concept assay for this method was carried out using existing hardware on the International Space Station and the results were read instantly by an astronaut through a simple color change of the reaction mixture. LAMP offers a novel platform for on‐orbit DNA‐based diagnostics that can be deployed on the International Space Station and to the broader benefit of space programs.

【 授权许可】

Unknown