Authentication of Illicium verum using a DNA barcode psbA-trnH

Dynamic Chiropractic

关键词: Illicium verum;    psbA-trnH;    identification;    DNA barcoding;    Illiciaceae;    medicinal material.;   

DOI  :  10.5897/JMPR11.1562

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The objective of this research was to test an appropriate DNA barcode from four candidate barcoding loci(matK, rbcL, psbA-trnHand ITS2) for discrimination betweenIllicium verumand its congeneric toxic adulterants in trade. Twenty fresh samples from 8 species of the generaIlliciumwere collected from four areas of China, and two samples of medicinal material ofI. verumwere purchased respectively, from the market and pharmacy. In this study, PCR amplification success rate, sequencing efficiency, the intra- and inter-specific divergences, and DNA barcoding gap were employed to assess the ability of each barcode for the authenticationofI. verumand its adultrants. The results showed thatpsbA-trnHexhibited the highest inter-specific divergence among the four DNA barcoding sequences and the highest species identification efficiency at 100% both by BLAST1 and the nearest distance method. In addition, thepsbA-trnHquery sequences of the two dried fruits were compared with the reference sequence by BLAST1, they were identified asI. verum. Our findings supported the notion that thepsbA-trnHregion could distinguishI. verumfrom other adulterating species.

【 授权许可】

Unknown