Biofilm Formation in Klebsiella pneumoniae Bacteremia Strains Was Found to be Associated with CC23 and the Presence of wcaG

Frontiers in Cellular and Infection Microbiology

Sun, Xiang¹  Yu, Zhi-jian¹  Zheng, Jin-xin¹  Chen, Zhong¹  Deng, Qi-wen²  Yang, Si-yu²  Qu, Di³  Chen, Chen⁴  Wu, Yang⁴  Li, Duo-yun⁴  Jin, Jia-lin⁵  Lin, Fo-jun⁵  Yao, Wei-ming⁵  Lin, Zhi-wei⁵ 

[1] Department of Infectious Diseases and the Key Laboratory of Endogenous Infection, Shenzhen Nanshan People'Department of Infectious Diseases, Huashan Hospital, Fudan University, China;Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Science and Institutes of Biomedical Sciences, Shanghai Medical College of Fudan University, China;Quality Center of Hospital-Acquired Infection and Control, China;s Hospital of Shenzhen University, China

关键词: Klebsiella pneumoniae;    Bacteremia;    Biofilm formation;    virulence genes;    Multilocus Sequence Typing;   

DOI  :  10.3389/fcimb.2018.00021

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Klebsiella pneumoniae bacteremia biofilm traits and distribution characteristics have not been clarified. This study aimed to determine the prevalence and characteristics of K. pneumoniae bacteremia biofilm formation (BF) and to explore the virulence factors associated with K. pneumoniae BF. A total of 250 K. pneumoniae bacteremia isolates were collected from patients in Shenzhen and Shanghai, China. Virulence genes in their genomes were detected by PCR. The isolates were subjected to multilocus sequence typing (MLST) and clonal complex (CC) classification based on housekeeping genes. Biofilms were detected by crystal violet staining. Greater BF was observed in isolates from young adults (<40 years old) than in those from seniors (≥65 years old; P=0.002). MLST yielded 65 different sequence types (STs), with the most represented STs being ST11, ST23, and ST65, and the main CCs were CC23 and CC65; CC23 isolates exhibited greater BF than CC65 or ST11 isolates (both P<0.001). BF was more pronounced among magA(K1), aero+, rmpA+, rmpA2+, allS+, wcaG+, and iutA+ isolates than in isolates that were negative for these virulence factors. Multivariate regression analysis revealed only wcaG as an independent risk factor for BF (odds ratio 11.426, P<0.001), and BF was decreased when wcaG was silenced by antisense RNA. In conclusion, BF in K. pneumoniae bacteremia isolates was found to be associated with CC23 classification and the presence of the wcaG virulence factor gene.

【 授权许可】

CC BY