Identification of the genus Epimedium with DNA barcodes

Dynamic Chiropractic

Yu Jiang¹  Chun-bang Ding² 

[1] College of Biology and Science, Sichuan Agricultural University, Yaan 625014, China.;Key Laboratory of Crop Genetic Resources and Improvement Ministry of Education, Sichuan Agricultural University, Yaan 625014, China.

关键词: Epimedium;    DNA barcode;    ITS;    rbcL;    matK;    psbA-trnH.;   

DOI  :  

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The traditional identification forEpimediumis inadequate due to the morphological variation and complicated procedure. DNA barcoding is a new technique for species identification by a standard DNA region. However, there has been no consensus emerged for plant barcoding due to the low rates of evolution in plastid DNA. In this paper, we compared the performance of four candidate DNA regions (psbA-trnH, ITS,rbcL,matK) in ten species ofEpimedium.To select a suitable barcode, ranking criteria included: the success efficiency of polymerase chain reaction (PCR) amplification and sequencing; genetic divergences; the barcoding gap. ThepsbA-trnH represented easy amplification, high level of interspecific divergence and well-separated barcoding gap. The other three loci (ITS,rbcL,matK) was inadequate for application. We concluded that thepsbA-trnH region was the better potentially DNA barcode for the genusEpimedium.

【 授权许可】

Unknown