Effect of salicylic acid (SA) and gibberellic acid (GA3) pre-soaking on seed germination of stevia (Stevia rebaudiana) under salt stress

Dynamic Chiropractic

关键词: SteviaStevia rebaudiana;    seedgermination;    NaCl;    NaHCO3;    salicylic acid (SA);    gibberellic acid (GA3).;   

DOI  :  10.5897/JMPR11.1106

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

The role ofsalicylic acid(SA) andgibberellic acid(GA3)pre-soaking were examined on stevia(Stevia rebaudiana) seed germination underNaCl or NaHCO3stress. A set of seeds was pre-soaked inH2O,another set of seeds in 0, 0.1, 0.5, 1.0, 5.0 and 10.0 mM SA and another set in 200 ppm GA3for 24 h.Seeds werethenplacedinPetri-disheswith 5 ml of 0, 50, 100, 200 and 300 mM NaCl or NaHCO3. Under the highest salinity condition no germination was observed.Seed germination was 65% in H2O, 0, 5 and1 mM SA, 33% in5 mM SA,12, 5% in10 mM SA,90% in 200 ppm GA344% in 50 mM NaCl, 14% in 100 mM NaCl, 40% in 50 mM NaHCO3, 11% in 100 mM NaHCO3. The germination velocity was 10.50 in H2O, 6.50 in 1mM SA,15.00 in 200 ppm GA3, 7.00 in 50 mM NaCl, 1.50 in 100 mM NaCl, 5.00 in 50 mM NaHCO3, 1.00 in 100 mM NaHCO3. Seed germination and velocity in NaCl was higher than the corresponding ones in NaHCO3.By pre-soaking in 0.5, 1.0 mM SA the seed germinationwas as much as in H2O, whilein 5.0, 10.0 mM SAthey were lessthan in H2O. By pre-soaking inall SAconcentrations,the 50 or 100 mM NaCl and also in NaHCO3, thisresulted in the reduction of germination in all combinations, when compared to their respective one in only 50 or 100 mM NaCl. In all SA concentrations the velocity was lower than the corresponding one in H2O.Highest velocitywas observed in 200 ppm GA3.

【 授权许可】

Unknown