Comparative Proteomics Reveals Differences in Host-Pathogen Interaction between Infectious and Commensal Relationship with Campylobacter jejuni

Frontiers in Cellular and Infection Microbiology

Aguilar, Carmen²  ctor²  nez-Marí²  Aylló³  Moreno, Angela⁴  Zaldí⁴  n, Á⁴  n, Nieves^{5  6}  Argü⁶  pez, Sara⁶  Villar, Margarita⁷  ello, Hé⁷  Garrido, Juan J.⁷  De La Fuente, José⁸  var-Ló⁸  ngeles⁸  Jimé⁹ 

[1] Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, USA;Grupo de GenóSaBio, Instituto de Investigaciómica y Mejora Animal, Departamento de Genén en Recursos Cinegérdoba, Córdoba, Spain;tica, Facultad de Veterinaria, Universidad de Cóticos (CSIC-UCLM-JCCM), Ciudad Real, Spain

关键词: human;    pig;    intestinal epithelial cells;    SWATH-MS;    Immunity;    Infection;   

DOI  :  10.3389/fcimb.2017.00145

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Campylobacter jejuni is the leading food-borne poisoning in industrialized countries. While the bacteria causes disease in humans, it merely colonizes the gut in poultry or pigs, where seems to establish a commensal relationship. Until now, few studies have been conducted to elucidate the relationship between C. jejuni and its different host. In this work, a comparative proteomics approach was used to identify the underlying mechanisms involved in the divergent outcome following C. jejuni infection in human and porcine host. Human (INT-407) and porcine (IPEC-1) intestinal cell lines were infected by C. jejuni for 3 h (T3h) and 24 h (T24h). C. jejuni infection prompted an intense inflammatory response at T3h in human intestinal cells, mainly characterized by expression of proteins involved in cell spreading, cell migration and promotion of reactive oxygen species (ROS). Proteomic analysis evidenced significantly regulated biofunctions in human cells related with engulfment and endocytosis, and supported by canonical pathways associated to infection such as caveolar- and clathrin-mediated endocytosis signaling. In porcine IPEC-1 cells, inflammatory response as well as signaling pathways that control cellular functions such as cell migration, endocytosis and cell cycle progression resulted downregulated. These differences in the host response to infection were supported by the different pattern of adhesion and invasion proteins expressed by C. jejuni in human and porcine cells. No marked differences in expression of virulence factors involved in adaptive response and iron acquisition functions were observed. Therefore, the results of this study suggest that both host and pathogen factors are responsible for commensal or infectious character of C. jejuni in different hosts.

【 授权许可】

CC BY