Resident Endothelial Progenitor Cells from Human Placenta have Greater Vasculogenic Potential than Circulating Endothelial Progenitor Cells from Umbilical Cord Blood

Cell Medicine

Oscar Moreno¹  Brian M. Rapp¹  Richard H. Ofstein¹  Dana A. Booth¹  Michael C. Dalsing¹  M. Reza Saadatzedeh²  Janak R. Bhavsar²  Michael P. Murphy³  Mervin C. Yoder⁴  David A. Ingram⁴  Dmitry O. Traktuev⁵  Keith L. March⁶  Zachary S. Tempel⁷  Peter Morone⁷ 

[1] *Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA;*Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA†Indiana Center for Vascular Biology and Medicine, Indiana University School of Medicine, Indianapolis, IN, USA;*Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA†Indiana Center for Vascular Biology and Medicine, Indiana University School of Medicine, Indianapolis, IN, USA**Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA;¶Department of Pediatrics, Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA#Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA;†Indiana Center for Vascular Biology and Medicine, Indiana University School of Medicine, Indianapolis, IN, USA§Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA;†Indiana Center for Vascular Biology and Medicine, Indiana University School of Medicine, Indianapolis, IN, USA§Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA**Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA;‡Indiana University School of Medicine, Indianapolis, IN, USA

关键词: Vascular endothelial cells;    Endothelial progenitor cells;    Placenta;    Vasculogenesis;    Pluripotent stem cells;   

DOI  :  10.3727/215517911X617888

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood (CBECFCs) are highly proliferative and form blood vessels in vivo. The purpose of this investigation was to isolate and characterize a population of resident ECFCs from the chorionic villi of term human placenta and provide a comparative analysis of their proliferative and vasculogenic potential with CBECFCs. ECFCs were isolated from umbilical cord blood and chorionic villi from placentas obtained by caesarean deliveries. Placental ECFCs (PECFCs) expressed CD144, CD31, CD105, and KDR and were negative for CD45 and CD34, consistent with other ECFC phenotypes. PECFCs were capable of 28.6 ± 6.0 population doublings before reaching senescence (vs. 47.4 ± 3.2 for CBECFCs, p < 0.05, n = 4). In single cell assays, 46.5 ± 1.2% underwent at least one division (vs. 51.0 ± 1.8% of CBECFCs, p = 0.07, n = 6), and of those dividing PECFCs, 71.8 ± 0.9% gave rise to colonies of >500 cells (highly proliferative potential clones) over 14 days (vs. 69.4 ± 0.7% of CBECFCs, p = 0.07, n = 9). PECFCs formed 5.2 ± 0.8 vessels/mm2 in collagen/fibronectin plugs implanted into non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice, whereas CBECFCs formed only 1.7 ± 1.0 vessels/mm2 (p < 0.05, n = 4). This study demonstrates that circulating CBECFCs and resident PECFCs are identical phenotypically and contain equivalent quantities of high proliferative potential clones. However, PECFCs formed significantly more blood vessels in vivo than CBECFCs, indicating that differences in vasculogenic potential between circulating and resident ECFCs exist.

【 授权许可】

CC BY