Double-bilayer: a new phase formed by lysophospholipids and the corresponding fatty acid

Quimica nova

Rapp, Gert¹  Funari, Sérgio S.¹  Richter, Frank² 

[1] DESY;DESY, Garching, Germany

关键词: PLA2;    lipid bilayer;    X-rays.;   

DOI  :  10.1590/S0100-40422009000400015

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The product ofcatalytic activity of the enzyme phospholipase A2, which resembles the coreunit of animal toxins, on phospholipids is a 1:1 mixture of lysolipid and fattyacid. This mixture was studied by time-resolved simultaneous small- and wideangle x-ray diffraction over the temperature range from 23 to 53.5ºC. An unusuallylarge lamellar structure was observed, with d = 11 nm, contradictingthe complex functional dimer model between lysolipid and fatty acid. It canbe explained by formation of a "double-bilayer", a new phase consisting of twodifferent bilayers, one formed by lysophospholipid and other by fatty acid,bound together by head group interactions. Its strucutre was confirmed by simulationsof the X-ray scattering pattern.

【 授权许可】

CC BY