HIV-1 Transmitting Couples Have Similar Viral Load Set-Points in Rakai, Uganda

PLoS Pathogens

Aleisha Collinson-Streng¹  Christl A. Donnelly²  David Serwadda²  T. Déirdre Hollingsworth²  George Shirreff³  Ronald H. Gray³  Christophe Fraser⁴  Thomas C. Quinn⁴  Fred Nalugoda⁴  William P. Hanage⁴  Noah Kiwanuka⁴  Oliver Laeyendecker⁵  Maria J. Wawer⁶  Victor Ssempijja⁶ 

[1] Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America;MRC Centre for Outbreak Analysis and Modelling, Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom;National Institute of Allergy and Infectious Diseases, National Institutes of Health, Baltimore, Maryland, United States of America;Rakai Health Science Program, Entebbe, Uganda;School of Medicine, Johns Hopkins University, Baltimore, Maryland, United States of America;School of Public Health, Makerere University, Kampala, Uganda

关键词: Viral load;    HIV-1;    Phylogenetic analysis;    Sequence analysis;    Genetic linkage;    Genetic epidemiology;    HIV;    Microbial genetics;   

DOI  :  10.1371/journal.ppat.1000876

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

It has been hypothesized that HIV-1 viral load set-point is a surrogate measure of HIV-1 viral virulence, and that it may be subject to natural selection in the human host population. A key test of this hypothesis is whether viral load set-points are correlated between transmitting individuals and those acquiring infection. We retrospectively identified 112 heterosexual HIV-discordant couples enrolled in a cohort in Rakai, Uganda, in which HIV transmission was suspected and viral load set-point was established. In addition, sequence data was available to establish transmission by genetic linkage for 57 of these couples. Sex, age, viral subtype, index partner, and self-reported genital ulcer disease status (GUD) were known. Using ANOVA, we estimated the proportion of variance in viral load set-points which was explained by the similarity within couples (the ‘couple effect’). Individuals with suspected intra-couple transmission (97 couples) had similar viral load set-points (p = 0.054 single factor model, p = 0.0057 adjusted) and the couple effect explained 16% of variance in viral loads (23% adjusted). The analysis was repeated for a subset of 29 couples with strong genetic support for transmission. The couple effect was the major determinant of viral load set-point (p = 0.067 single factor, and p = 0.036 adjusted) and the size of the effect was 27% (37% adjusted). Individuals within epidemiologically linked couples with genetic support for transmission had similar viral load set-points. The most parsimonious explanation is that this is due to shared characteristics of the transmitted virus, a finding which sheds light on both the role of viral factors in HIV-1 pathogenesis and on the evolution of the virus.

【 授权许可】

CC BY