PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE

Journal of The Chilean Chemical Society

SZYMANSKA, RENATA¹  STRZALKA, KAZIMIERZ¹  STOJAKOWSKA, ANNA²  AVELLO, MARCIA³  SUWALSKY, MARIO³  VILLENA, FERNANDO³  OBREQUE, JOSE³ 

[1] Jagiellonian University, Kraków, Poland;Polish Academy of Sciences, Krakow, Poland;University of Concepción, Concepción, Chile

关键词: Philesia magellanica;    Coicopihue;    Plant extract;    Antioxidant;    Human erythrocyte;    Cell membrane.;   

DOI  :  10.4067/S0717-97072015000200016

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

Philesia magellanica (P. magellanica) is a plant collected in the Chilean Patagonia. Its antioxidant properties were assessed in human erythrocytes exposed in vitro to oxidative stress induced by HClO. Scanning electron microscopy (SEM) observations showed that HClO induced a morphological alteration in the red blood cells from a normal discoid to a spherocytic form, and cells of unequal size. However, a concentration as low as 1 μM gallic acid equivalents (GAE) of P. magellanica aqueous extract neutralized the change effects of 50 μΜ HClO. On the other hand, 20 μΜ (GAE) of the extract considerably reduced the deleterious capacity of 0.25 mM HClO to induce hemolysis in red blood cells. In addition, X-ray diffraction experiments were performed in molecular models of the human erythrocyte membrane. These consisted in multilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), classes of lipids preferentially located in the outer and inner monolayers, respectively of the human erythrocyte membrane. It was observed that P. magellanica only interacted with DMPC affecting its multilayer structure. It was also observed that 0.1 μM (GAE) of P. magellanica neutralized the structural perturbation induced by 0.05 and 0.5 mM HClO. These experiments confirmed the antioxidant properties of P. magellanica aqueous extracts.

【 授权许可】

Unknown