Lipids in Health and Disease | |
Guaraná (Paullinia cupana Kunth) effects on LDL oxidation in elderly people: an in vitro and in vivo study | |
Felix Alexandre Antunes Soares3 Leila Suleiman3 Ivana Beatrice Mânica da Cruz1 Euler Esteves Ribeiro2 Edovando José Flores da Rosa3 Rômulo Pillon Barcelos3 Rafael de Lima Portella3 | |
[1] Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS, Brazil;Universidade Aberta da Terceira Idade, Universidade do Estado do Amazonas, Amazonas, Brazil;Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus UFSM, Santa Maria, RS, Brazil | |
关键词: Plant extract; Antioxidant; Humans; Lipoperoxidation; | |
Others : 1160086 DOI : 10.1186/1476-511X-12-12 |
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received in 2012-10-23, accepted in 2013-01-31, 发布年份 2013 | |
【 摘 要 】
Background
Previous experimental investigations have suggested that guaraná (Paullinia cupana Kunth, supplied by EMBRAPA Oriental) consumption is associated with a lower prevalence of cardiovascular metabolic diseases and has positive effects on lipid metabolism, mainly related to low density lipoprotein (LDL) levels. As LDL oxidation is an important initial event in the development of atherosclerosis, we performed in vitro and in vivo studies to observe the potential effects of guaraná on LDL and serum oxidation.
Methods
The in vivo protocol was performed using blood samples from 42 healthy elderly subjects who habitually ingested guaraná (GI) or never ingested guaraná (NG). The formation of conjugated dienes (CDs) was analyzed from serum samples. The in vitro protocols were performed using LDL obtained from 3 healthy, non-fasted, normolipidemic voluntary donors who did not habitually ingest guaraná in their diets. The LDL samples were exposed to 5 different guaraná concentrations (0.05, 0.1, 0.5, 1, and 5 μg/mL).
Results
GI subjects demonstrated lower LDL oxidation than did NG subjects (reduction of 27%, p < 0.0014), independent of other variables. In the GI group the total polyphenols was positively associated with LDL levels. Also, guaraná demonstrated a high antioxidant activity in vitro, mainly at concentrations of 1 and 5 μg/mL, demonstrated by suppression of CDs and TBARS productions, tryptophan destruction and high TRAP activity.
Conclusions
Guaraná, similar to other foods rich in caffeine and catechins such as green tea, has some effect on LDL oxidation that could partially explain the protective effects of this food in cardiometabolic diseases.
【 授权许可】
2013 Portella et al; licensee BioMed Central Ltd.
【 预 览 】
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