Bacillus anthracis Secretes Proteins That Mediate Heme Acquisition from Hemoglobin

PLoS Pathogens

Gabriella Garufi¹  Olaf Schneewind¹  Anthony W. Maresso¹ 

[1] Department of Microbiology, University of Chicago, Chicago, Illinois, United States of America

关键词: Heme;    Hemoglobin;    Bacillus anthracis;    Protein domains;    Cell walls;    Glutathione chromatography;    Membrane proteins;    Sediment;   

DOI  :  10.1371/journal.ppat.1000132

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Acquisition of iron is necessary for the replication of nearly all bacterial pathogens; however, iron of vertebrate hosts is mostly sequestered by heme and bound to hemoglobin within red blood cells. In Bacillus anthracis, the spore-forming agent of anthrax, the mechanisms of iron scavenging from hemoglobin are unknown. We report here that B. anthracis secretes IsdX1 and IsdX2, two NEAT domain proteins, to remove heme from hemoglobin, thereby retrieving iron for bacterial growth. Unlike other Gram-positive bacteria, which rely on cell wall anchored Isd proteins for heme scavenging, B. anthracis seems to have also evolved NEAT domain proteins in the extracellular milieu and in the bacterial envelope to provide for the passage of heme.

【 授权许可】

CC BY