Salmonella Uses Energy Taxis to Benefit from Intestinal Inflammation

PLoS Pathogens

Joseph M. Russell¹  Charles L. Bevins¹  Sebastian E. Winter¹  Maria G. Winter¹  Sean-Paul Nuccio¹  Christopher A. Lopez¹  Mariana N. Xavier¹  Torsten Sterzenbach¹  Andreas J. Bäumler¹  Fabian Rivera-Chávez¹  Renée M. Tsolis¹  Richard C. Laughlin²  L. Garry Adams²  Sara D. Lawhon²  Rasika Harshey³ 

[1] Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, California, United States of America;Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America;Section of Molecular Genetics and Microbiology, School of Biological Sciences, University of Texas at Austin, Austin, Texas, United States of America

关键词: Salmonella typhimurium;    Nitrates;    Inflammation;    Polymerase chain reaction;    Colitis;    Respiratory infections;    Chemotaxis;    Electron acceptors;   

DOI  :  10.1371/journal.ppat.1003267

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Chemotaxis enhances the fitness of Salmonella enterica serotype Typhimurium (S. Typhimurium) during colitis. However, the chemotaxis receptors conferring this fitness advantage and their cognate signals generated during inflammation remain unknown. Here we identify respiratory electron acceptors that are generated in the intestinal lumen as by-products of the host inflammatory response as in vivo signals for methyl-accepting chemotaxis proteins (MCPs). Three MCPs, including Trg, Tsr and Aer, enhanced the fitness of S. Typhimurium in a mouse colitis model. Aer mediated chemotaxis towards electron acceptors (energy taxis) in vitro and required tetrathionate respiration to confer a fitness advantage in vivo. Tsr mediated energy taxis towards nitrate but not towards tetrathionate in vitro and required nitrate respiration to confer a fitness advantage in vivo. These data suggest that the energy taxis receptors Tsr and Aer respond to distinct in vivo signals to confer a fitness advantage upon S. Typhimurium during inflammation by enabling this facultative anaerobic pathogen to seek out favorable spatial niches containing host-derived electron acceptors that boost its luminal growth.

【 授权许可】

CC BY