Eight RGS and RGS-like Proteins Orchestrate Growth, Differentiation, and Pathogenicity of Magnaporthe oryzae

PLoS Pathogens

Ping Wang¹  Zhongqiang Qi²  Qian Huang²  Xin Zhang²  Haifeng Zhang²  Jiansheng Wang²  Kaiyue Liu²  Wei Tang²  Xiaobo Zheng²  Yue Chen²  Zhengguang Zhang²  Zhengyi Wang³  Xia Yan³ 

[1] Department of Pediatrics and the Research Institute for Children, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America;Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China;State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Huajiachi Campus, Hangzhou, China

关键词: Appressoria;    G-protein signaling;    Pathogenesis;    Mutant strains;    Fungal pathogens;    Laccases;    Rice blast fungus;    Peroxidases;   

DOI  :  10.1371/journal.ppat.1002450

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

A previous study identified MoRgs1 as an RGS protein that negative regulates G-protein signaling to control developmental processes such as conidiation and appressorium formation in Magnaporthe oryzae. Here, we characterized additional seven RGS and RGS-like proteins (MoRgs2 through MoRgs8). We found that MoRgs1 and MoRgs4 positively regulate surface hydrophobicity, conidiation, and mating. Indifference to MoRgs1, MoRgs4 has a role in regulating laccase and peroxidase activities. MoRgs1, MoRgs2, MoRgs3, MoRgs4, MoRgs6, and MoRgs7 are important for germ tube growth and appressorium formation. Interestingly, MoRgs7 and MoRgs8 exhibit a unique domain structure in which the RGS domain is linked to a seven-transmembrane motif, a hallmark of G-protein coupled receptors (GPCRs). We have also shown that MoRgs1 regulates mating through negative regulation of Gα MoMagB and is involved in the maintenance of cell wall integrity. While all proteins appear to be involved in the control of intracellular cAMP levels, only MoRgs1, MoRgs3, MoRgs4, and MoRgs7 are required for full virulence. Taking together, in addition to MoRgs1 functions as a prominent RGS protein in M. oryzae, MoRgs4 and other RGS and RGS-like proteins are also involved in a complex process governing asexual/sexual development, appressorium formation, and pathogenicity.

【 授权许可】

CC BY