PLoS Pathogens | |
Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda | |
Sean Conlan1  W. Ian Lipkin1  Phenix-Lan Quan1  Robert Downing2  Jordan W. Tappero2  Samuel Okware3  Marina L. Khristova4  James A. Comer5  Jonathan S. Towner5  Serena A. Reeder5  Pierre E. Rollin5  Thomas G. Ksiazek5  César G. Albariño5  Stuart T. Nichol5  Tara K. Sealy5  Julius Lutwama6  Barnabas Bakamutumaho6  John Kayiwa6  | |
[1] Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America;Global AIDS Program, Centers for Disease Control and Prevention, Entebbe, Uganda;Ministry of Health, Republic of Uganda, Kampala, Uganda;Scientific Resources Program, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America;Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America;Uganda Virus Research Institute, Entebbe, Uganda | |
关键词: Reverse transcriptase-polymerase chain reaction; Viral genomics; DNA sequence analysis; Ebola virus; Côte d'Ivoire; Phylogenetic analysis; Sequence analysis; Epidemiology; | |
DOI : 10.1371/journal.ppat.1000212 | |
学科分类:生物科学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
Over the past 30 years, Zaire and Sudan ebolaviruses have been responsible for large hemorrhagic fever (HF) outbreaks with case fatalities ranging from 53% to 90%, while a third species, Côte d'Ivoire ebolavirus, caused a single non-fatal HF case. In November 2007, HF cases were reported in Bundibugyo District, Western Uganda. Laboratory investigation of the initial 29 suspect-case blood specimens by classic methods (antigen capture, IgM and IgG ELISA) and a recently developed random-primed pyrosequencing approach quickly identified this to be an Ebola HF outbreak associated with a newly discovered ebolavirus species (Bundibugyo ebolavirus) distantly related to the Côte d'Ivoire ebolavirus found in western Africa. Due to the sequence divergence of this new virus relative to all previously recognized ebolaviruses, these findings have important implications for design of future diagnostic assays to monitor Ebola HF disease in humans and animals, and ongoing efforts to develop effective antivirals and vaccines.
【 授权许可】
CC BY
【 预 览 】
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RO201902018531319ZK.pdf | 281KB | download |