Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda

PLoS Pathogens

Sean Conlan¹  W. Ian Lipkin¹  Phenix-Lan Quan¹  Robert Downing²  Jordan W. Tappero²  Samuel Okware³  Marina L. Khristova⁴  James A. Comer⁵  Jonathan S. Towner⁵  Serena A. Reeder⁵  Pierre E. Rollin⁵  Thomas G. Ksiazek⁵  César G. Albariño⁵  Stuart T. Nichol⁵  Tara K. Sealy⁵  Julius Lutwama⁶  Barnabas Bakamutumaho⁶  John Kayiwa⁶ 

[1] Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America;Global AIDS Program, Centers for Disease Control and Prevention, Entebbe, Uganda;Ministry of Health, Republic of Uganda, Kampala, Uganda;Scientific Resources Program, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America;Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America;Uganda Virus Research Institute, Entebbe, Uganda

关键词: Reverse transcriptase-polymerase chain reaction;    Viral genomics;    DNA sequence analysis;    Ebola virus;    Côte d'Ivoire;    Phylogenetic analysis;    Sequence analysis;    Epidemiology;   

DOI  :  10.1371/journal.ppat.1000212

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Over the past 30 years, Zaire and Sudan ebolaviruses have been responsible for large hemorrhagic fever (HF) outbreaks with case fatalities ranging from 53% to 90%, while a third species, Côte d'Ivoire ebolavirus, caused a single non-fatal HF case. In November 2007, HF cases were reported in Bundibugyo District, Western Uganda. Laboratory investigation of the initial 29 suspect-case blood specimens by classic methods (antigen capture, IgM and IgG ELISA) and a recently developed random-primed pyrosequencing approach quickly identified this to be an Ebola HF outbreak associated with a newly discovered ebolavirus species (Bundibugyo ebolavirus) distantly related to the Côte d'Ivoire ebolavirus found in western Africa. Due to the sequence divergence of this new virus relative to all previously recognized ebolaviruses, these findings have important implications for design of future diagnostic assays to monitor Ebola HF disease in humans and animals, and ongoing efforts to develop effective antivirals and vaccines.

【 授权许可】

CC BY