Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles

PLoS Pathogens

Ilaria Alberti¹  Stefano Tonti²  Bettina Tudzynski³  Il-Pyung Ahn⁴  Christian M. K. Sieber⁵  Sung-Hwan Yun⁶  Hee-Kyoung Kim⁶  Da-Woon Kim⁶  Martin Münsterkötter⁷  Ulrich Güldener⁷  Eva-Maria Niehaus⁸  Slavica Janevska⁸  Svetlana A. Kalinina⁹  Hans-Ulrich Humpf⁹  Birgit Arndt⁹  Petra M. Houterman^1,10 

[1] CREA-CIN Sede di Rovigo, Viale Giovanni Amendola, 82, 45100 Rovigo, Italy;CREA-SCS Sede di Bologna, Via di Corticella, 133, 40128 Bologna, Italy;Chair of Genome-oriented Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany;Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Republic of Korea;Department of Energy Joint Genome Institute, University of California, Walnut Creek, Berkeley, California;Department of Medical Biotechnology, Soonchunhyang University, Asan, Republic of Korea;Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Neuherberg, Germany;Institute of Biology and Biotechnology of Plants, Molecular Biology and Biotechnology of Fungi, Westfälische Wilhelms-Universität Münster, Münster, Germany;Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 45, Münster, Germany;University of Amsterdam, Swammerdam Institute for Life Sciences, Plant Pathology, Amsterdam, The Netherlands

关键词: Rice;    Fusarium;    Seedlings;    Genomics;    Gibberellins;    Comparative genomics;    Gene expression;    Polymerase chain reaction;   

DOI  :  10.1371/journal.ppat.1006670

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Fusarium fujikuroi causes bakanae (“foolish seedling”) disease of rice which is characterized by hyper-elongation of seedlings resulting from production of gibberellic acids (GAs) by the fungus. This plant pathogen is also known for production of harmful mycotoxins, such as fusarins, fusaric acid, apicidin F and beauvericin. Recently, we generated the first de novo genome sequence of F. fujikuroi strain IMI 58289 combined with extensive transcriptional, epigenetic, proteomic and chemical product analyses. GA production was shown to provide a selective advantage during infection of the preferred host plant rice. Here, we provide genome sequences of eight additional F. fujikuroi isolates from distant geographic regions. The isolates differ in the size of chromosomes, most likely due to variability of subtelomeric regions, the type of asexual spores (microconidia and/or macroconidia), and the number and expression of secondary metabolite gene clusters. Whilst most of the isolates caused the typical bakanae symptoms, one isolate, B14, caused stunting and early withering of infected seedlings. In contrast to the other isolates, B14 produced no GAs but high amounts of fumonisins during infection on rice. Furthermore, it differed from the other isolates by the presence of three additional polyketide synthase (PKS) genes (PKS40, PKS43, PKS51) and the absence of the F. fujikuroi-specific apicidin F (NRPS31) gene cluster. Analysis of additional field isolates confirmed the strong correlation between the pathotype (bakanae or stunting/withering), and the ability to produce either GAs or fumonisins. Deletion of the fumonisin and fusaric acid-specific PKS genes in B14 reduced the stunting/withering symptoms, whereas deletion of the PKS51 gene resulted in elevated symptom development. Phylogenetic analyses revealed two subclades of F. fujikuroi strains according to their pathotype and secondary metabolite profiles.

【 授权许可】

CC BY