Independent Bottlenecks Characterize Colonization of Systemic Compartments and Gut Lymphoid Tissue by Salmonella

PLoS Pathogens

Robert Geffers¹  Mikael Rhen²  Syed Fazle Rouf²  Chee Han Lim³  Benjamin Wahl³  Oliver Pabst³  Sabrina Voedisch³ 

[1] Department of Genome Analytics, Helmholtz Centre for Infection Research, Braunschweig, Germany;Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden;Institute of Immunology, Hannover Medical School, Hannover, Germany

关键词: Salmonellosis;    Salmonella;    Salmonella typhimurium;    Spleen;    Vaccination;    immunization;    Polymerase chain reaction;    Cloning;    Mammalian genomics;   

DOI  :  10.1371/journal.ppat.1004270

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Vaccination represents an important instrument to control typhoid fever in humans and protects mice from lethal infection with mouse pathogenic serovars of Salmonella species. Mixed infections with tagged Salmonella can be used in combination with probabilistic models to describe the dynamics of the infection process. Here we used mixed oral infections with tagged Salmonella strains to identify bottlenecks in the infection process in naïve and vaccinated mice. We established a next generation sequencing based method to characterize the composition of tagged Salmonella strains which offers a fast and reliable method to characterise the composition of genome-tagged Salmonella strains. We show that initial colonization of Salmonella was distinguished by a non-Darwinian selection of few bacteria setting up the infection independently in gut associated lymphoid tissue and systemic compartments. Colonization of Peyer's patches fuels the sustained spread of bacteria into mesenteric lymph nodes via dendritic cells. In contrast, infection of liver and spleen originated from an independent pool of bacteria. Vaccination only moderately reduced invasion of Peyer's patches but potently uncoupled bacterial populations present in different systemic compartments. Our data indicate that vaccination differentially skews the capacity of Salmonella to colonize systemic and gut immune compartments and provide a framework for the further dissection of infection dynamics.

【 授权许可】

CC BY