Complement as an Endogenous Adjuvant for Dendritic Cell-Mediated Induction of Retrovirus-Specific CTLs

PLoS Pathogens

Christoph Gassner¹  Zoltán Bánki²  Asim Ejaz²  Manfred P. Dierich²  Heribert Stoiber²  Doris Wilflingseder²  Verena Oberhauser²  Wilfried Posch²  Ulf Dittmer³  Kim J. Hasenkrug⁴  Suzanne Willey⁵ 

[1] Central Institute for Blood Transfusion & Immunological Department, Innsbruck, Tirol, Austria;Department of Hygiene, Microbiology and Social Medicine, Division of Virology, Innsbruck Medical University, Innsbruck, Tirol, Austria;Institute of Virology, University of Duisburg-Essen, Essen, Germany;Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, Hamilton, Montana, United States of America;MRC/UCL Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, London, United Kingdom

关键词: T cells;    Cytotoxic T cells;    HIV;    Complement system;    Opsonization;    Secretion;    Cell staining;    Spleen;   

DOI  :  10.1371/journal.ppat.1000891

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Previous studies have demonstrated the involvement of complement (C) in induction of efficient CTL responses against different viral infections, but the exact role of complement in this process has not been determined. We now show that C opsonization of retroviral particles enhances the ability of dendritic cells (DCs) to induce CTL responses both in vitro and in vivo. DCs exposed to C-opsonized HIV in vitro were able to stimulate CTLs to elicit antiviral activity significantly better than non-opsonized HIV. Furthermore, experiments using the Friend virus (FV) mouse model illustrated that the enhancing role of complement on DC-mediated CTL induction also occurred in vivo. Our results indicate that complement serves as natural adjuvant for DC-induced expansion and differentiation of specific CTLs against retroviruses.

【 授权许可】

CC BY