Chiral chromatographic separation of anesthetics from dilute and concentrated solutions under preparative scale

Quimica nova

Santana, Cesar Costapinto¹  Silva Jr., Ivanildo José da²  Barreto Jr., Amaro Gomes³ 

[1] Universidade Estadual de Campinas, Campinas, Brasil;Universidade Federal do Ceará, Fortaleza, Brasil;Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil

关键词: enantiomer separation;    chiral stationary phases;    liquid chromatography.;   

DOI  :  10.1590/S0100-40422009000200017

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

In this work the separation of the chiral anesthetic compounds ketamine and bupivacaine was development using two chiral stationary phases (CSP). Ketamine enantiomers were well separate in the polysaccharide-based CSP (microcrystalline cellulose triacetate - MCTA) while bupivacaine in the tartardiamide-based CSP (Kromasil CHI-TBB). In both cases, the effect of temperature was investigated under analytical conditions. An improvement in the separation performance with temperature was observed. Thermodynamic parameters were evaluated by the van't Hoff plot. We concluded that enthalpic effects controlled the retention in these chiral columns. The enantiomers of ketamine and bupivacaine were separated under overloaded conditions with a good performance.

【 授权许可】

CC BY