Expression of non-neuronal cholinergic system in maxilla of rat in vivo

Biological research: BR

Lue Wang¹  Xiaoxia Che²  Jie Guo⁴  Haihua Xu⁵ 

[1] Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, People’College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People’Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, People’Department of Orthodontics, School of Stomatology, Shandong University;s Republic of China

关键词: Non-neuronal cholinergic system;    Maxilla;    Real-Time PCR;    Immunohistochemistry;   

DOI  :  10.1186/0717-6287-47-72

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits α1, α2, α3, α5, α7, α10, β1, β2, β4, γ and mAChR subunits M1, M2, M3, M4, M5 were detected in rat’s maxilla. RNA of VAChT, AChE, nAChR subunits α2, β1, β4 and mAChR subunits M4 had abundant expression (2-ΔCt > 0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChRα7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor α7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells. ACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.

【 授权许可】

CC BY