Human Decidua-Derived Mesenchymal Cells Are a Promising Source for the Generation and Cell Banking of Human Induced Pluripotent Stem Cells

Cell Medicine

Atsuyo Yamamoto¹  Tomoko Shofuda¹  Yonehiro Kanemura²  Yoshikazu Sugimoto³  Sumiko Yoshitatsu⁴  Hiroshi Suemizu⁵  Masato Nakamura⁶  Mami Yamasaki⁷  Hayato Fukusumi⁸  Daisuke Kanematsu⁸  Miho Kusuda Furue⁹  Hideyuki Okano^1,10  Yohei Bamba^1,10  Wado Akamatsu^1,10  Yohei Okada^1,11  Arihiro Kohara^1,12 

[1] * Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Chuo-ku, Osaka, Japan;* Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Chuo-ku, Osaka, Japan## Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan;** Division of Chemotherapy, Faculty of Pharmacy, Keio University, Minato-ku, Tokyo, Japan;§ Department of Plastic Surgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan;¶ Biomedical Research Department, Central Institute for Experimental Animals, Kawasaki-ku, Kawasaki, Japan;¶ Biomedical Research Department, Central Institute for Experimental Animals, Kawasaki-ku, Kawasaki, Japan# Department of Pathology and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan;¶¶ Division of Molecular Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan## Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan*** Department of Pediatric Neurosurgery, Takatsuki General Hospital, Takatsuki, Osaka, Japan;† Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan;†† Laboratory of Stem Cell Cultures, Laboratory of Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan;‡ Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan;‡ Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan§§ Kanrinmaru-Project, School of Medicine, Keio University, Tokyo, Japan;‡‡ JCRB Cell Bank, Laboratory of Cell Cultures, Research on Disease Bioresources, National Institute of Biomedical Innovation, Osaka, Japan

关键词: Induced pluripotent stem cells (iPSCs);    Decidua;    Mesenchymal cells;    X-chromosome inactivation;   

DOI  :  10.3727/215517912X658918

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Placental tissue is a biomaterial with remarkable potential for use in regenerative medicine. It has a three-layer structure derived from the fetus (amnion and chorion) and the mother (decidua), and it contains huge numbers of cells. Moreover, placental tissue can be collected without any physical danger to the donor and can be matched with a variety of HLA types. The decidua-derived mesenchymal cells (DMCs) are highly proliferative fibroblast-like cells that express a similar pattern of CD antigens as bone marrow-derived mesenchymal cells (BM-MSCs). Here we demonstrated that induced pluripotent stem (iPS) cells could be efficiently generated from DMCs by retroviral transfer of reprogramming factor genes. DMC-hiPS cells showed equivalent characteristics to human embryonic stem cells (hESCs) in colony morphology, global gene expression profile (including human pluripotent stem cell markers), DNA methylation status of the OCT3/4 and NANOG promoters, and ability to differentiate into components of the three germ layers in vitro and in vivo. The RNA expression of XIST and the methylation status of its promoter region suggested that DMC-iPSCs, when maintained undifferentiated and pluripotent, had three distinct states: (1) complete X-chromosome reactivation, (2) one inactive X-chromosome, or (3) an epigenetic aberration. Because DMCs are derived from the maternal portion of the placenta, they can be collected with the full consent of the adult donor and have considerable ethical advantages for cell banking and the subsequent generation of human iPS cells for regenerative applications.

【 授权许可】

CC BY