Zika Virus infection of rhesus macaques leads to viral persistence in multiple tissues

PLoS Pathogens

Clayton A. Wiley¹  William B. Messer²  Tonya Swanson³  Michael K. Axthelm⁴  Alfred W. Legasse⁴  Rhonda MacAllister⁴  Jon D. Hennebold⁵  Peta L. Grigsby⁵  Lois M. A. Colgin⁶  Rebecca M. Ducore⁶  Jay A. Nelson⁷  Alec J. Hirsch⁸  Christopher J. Parkins⁸  Victor R. DeFilippis⁸  Patricia P. Smith⁸  Michael Denton⁸  Daniel N. Streblow⁸  Rebecca M. Broeckel⁸  Craig Kreklywich⁸  Jessica L. Smith⁸  Nicole N. Haese⁸ 

[1] Clinical Medicine Unit, Division of Comparative Medicine, Oregon National Primate Research Center, Beaverton, Oregon, United States of America;Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon, United States of America;Department of Obstetrics & Gynecology, Oregon Health and Science University, Portland, Oregon, United States of America;Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon, United States of America;Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon, United States of America;Pathology Services Unit, Division of Comparative Medicine, Oregon National Primate Research Center, Beaverton, Oregon, United States of America;UPMC Presbyterian Hospital, Division of Neuropathology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America;Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, United States of America

关键词: Zika virus;    T cells;    Lymph nodes;    Enzyme-linked immunoassays;    B cells;    Blood plasma;    Antibodies;    Urine;   

DOI  :  10.1371/journal.ppat.1006219

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Zika virus (ZIKV), an emerging flavivirus, has recently spread explosively through the Western hemisphere. In addition to symptoms including fever, rash, arthralgia, and conjunctivitis, ZIKV infection of pregnant women can cause microcephaly and other developmental abnormalities in the fetus. We report herein the results of ZIKV infection of adult rhesus macaques. Following subcutaneous infection, animals developed transient plasma viremia and viruria from 1–7 days post infection (dpi) that was accompanied by the development of a rash, fever and conjunctivitis. Animals produced a robust adaptive immune response to ZIKV, although systemic cytokine response was minimal. At 7 dpi, virus was detected in peripheral nervous tissue, multiple lymphoid tissues, joints, and the uterus of the necropsied animals. Notably, viral RNA persisted in neuronal, lymphoid and joint/muscle tissues and the male and female reproductive tissues through 28 to 35 dpi. The tropism and persistence of ZIKV in the peripheral nerves and reproductive tract may provide a mechanism of subsequent neuropathogenesis and sexual transmission.

【 授权许可】

CC BY