Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 from Bothrops atrox snake venom

Journal of venomous animals and toxins

Danilo L. Menaldo¹  Adé²  lia C. O. Cintra³  Anna L. Jacob-Ferreira⁴  Suely V. Sampaio⁵  Carolina P. Bernardes⁶ 

[1] Departamento de Anágicas e Bromatológicas, Faculdade de Ciêlises Clíncias Farmacênicas, Toxicolóo Paulo, (USP), Ribeirão Preto, Brasil;o Preto, Universidade de Sãuticas de Ribeirã

关键词: Snake venoms;    Bothrops atrox;    Toxins;    Metalloprotease;    Phospholipase A2;    Isolation;    Characterization;    Chromatography;   

DOI  :  10.1186/s40409-015-0027-6

学科分类：药理学

来源: BioMed Central

【 摘 要 】

Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA2) from Bothrops atrox venom, and biochemically characterize these molecules to enable future functional studies. To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of the molecules was confirmed by RP-HPLC and SDS-PAGE. The enzymes were characterized by determining their molecular masses, isoelectric points, specific functional activity and partial amino acid sequencing. The metalloprotease presented molecular mass of 22.9 kDa and pI 7.4, with hemorrhagic and fibrin(ogen)olytic activities, and its partial amino acid sequence revealed high similarity with other P-I class metalloproteases. These results suggest that the isolated metalloprotease is Batroxase, a P-I metalloprotease previously described by our research group. The phospholipase A2 showed molecular mass of 13.7 kDa and pI 6.5, with high phospholipase activity and similarity to other acidic PLA2s from snake venoms. These data suggest that the acidic PLA2 is a novel enzyme from B. atrox venom, being denominated BatroxPLA2. The present study successfully standardized a simple methodology to isolate the metalloprotease Batroxase and the acidic PLA2 BatroxPLA2 from the venom of B. atrox, consisting mainly of classical chromatographic processes. These two enzymes will be used in future studies to evaluate their effects on the complement system and the inflammatory process, in addition to the thrombolytic potential of the metalloprotease.

【 授权许可】

CC BY