Live Imaging of Influenza Infection of the Trachea Reveals Dynamic Regulation of CD8+ T Cell Motility by Antigen

PLoS Pathogens

Emma Reilly¹  Kris Lambert Emo¹  Scott Gerber¹  Minsoo Kim¹  Young-min Hyun²  Christopher Barilla²  David J. Topham²  Deborah Fowell² 

[1] David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America;Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America

关键词: T cells;    Trachea;    Cytotoxic T cells;    Cell motility;    Influenza;    Respiratory infections;    Pathogen motility;    Influenza viruses;   

DOI  :  10.1371/journal.ppat.1005881

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

During a primary influenza infection, cytotoxic CD8+ T cells need to infiltrate the infected airways and engage virus-infected epithelial cells. The factors that regulate T cell motility in the infected airway tissue are not well known. To more precisely study T cell infiltration of the airways, we developed an experimental model system using the trachea as a site where live imaging can be performed. CD8+ T cell motility was dynamic with marked changes in motility on different days of the infection. In particular, significant changes in average cell velocity and confinement were evident on days 8–10 during which the T cells abruptly but transiently increase velocity on day 9. Experiments to distinguish whether infection itself or antigen affect motility revealed that it is antigen, not active infection per se that likely affects these changes as blockade of peptide/MHC resulted in increased velocity. These observations demonstrate that influenza tracheitis provides a robust experimental foundation to study molecular regulation of T cell motility during acute virus infection.

【 授权许可】

CC BY