Viral Interference with DNA Repair by Targeting of the Single-Stranded DNA Binding Protein RPA

PLoS Pathogens

Ole Gjoerup¹  Pubali Banerjee¹  Brian S. Schaffhausen²  Rowena deJesus³ 

[1] Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, Massachusetts, United States of America;Molecular Oncology Research Institute, Tufts Medical Center, Boston, Massachusetts, United States of America;Program in Molecular Microbiology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America

关键词: DNA damage;    DNA repair;    Apoptosis;    DNA replication;    SV40;    DNA-binding proteins;    Fluorescence imaging;    Homologous recombination;   

DOI  :  10.1371/journal.ppat.1003725

学科分类：生物科学（综合）

来源: Public Library of Science

【 摘 要 】

Correct repair of damaged DNA is critical for genomic integrity. Deficiencies in DNA repair are linked with human cancer. Here we report a novel mechanism by which a virus manipulates DNA damage responses. Infection with murine polyomavirus sensitizes cells to DNA damage by UV and etoposide. Polyomavirus large T antigen (LT) alone is sufficient to sensitize cells 100 fold to UV and other kinds of DNA damage. This results in activated stress responses and apoptosis. Genetic analysis shows that LT sensitizes via the binding of its origin-binding domain (OBD) to the single-stranded DNA binding protein replication protein A (RPA). Overexpression of RPA protects cells expressing OBD from damage, and knockdown of RPA mimics the LT phenotype. LT prevents recruitment of RPA to nuclear foci after DNA damage. This leads to failure to recruit repair proteins such as Rad51 or Rad9, explaining why LT prevents repair of double strand DNA breaks by homologous recombination. A targeted intervention directed at RPA based on this viral mechanism could be useful in circumventing the resistance of cancer cells to therapy.

【 授权许可】

CC BY